|Event Title||First Global Alliance for Research on Avian Diseases (GARAD) Conference|
|Event Date & Time||On Wed, 01 Jul 2015 at 11:35:00 - 11:55:00|
|Venue||Edmond J. Safra Lecture Theatre|
|Abstract Title||Novel Nanoparticle-based vaccine induces specific neutralizing antibodies against Bird Flu|
|Affiliations||1Department of Pathobiology and Veterinary Science and Department of Molecular Cell Biology2, University of Connecticut, Storrs, CT 06269, USA|
Designing an effective and safe vaccine against bird flu is challenging due to continuous and rapid changes in the genome of avian influenza virus (AIV). We designed a novel nanoparticle platform called self-assembled peptide nanoparticle (SAPN) to deliver antigenic epitopes in their native conformations to elicit strong immune responses, and may lead to the development of universal influenza vaccines. The novel nanoparticle immunogens with built-in flagellin (Hel C-Flagellin NP) or not (Hel C NP) were administered to groups of specific pathogen free (SPF) chickens. Chickens vaccinated with Hel C-Flagellin NP produced 128-fold higher sera antibody titers detected by ELISA, as compared with chickens administered HelC NP. Avidity of neutralizing antibodies against virus infection in vitro increased 23-fold after vaccination of SANP with the aid of its built-in flagellin. Hel C-Flagellin. These results demonstrated that this Flagellin built-in Hel C novel nanoparticle-based vaccine display excellent immunogenicity without co-administration of a commercial adjuvant.