|Event Title||First Global Alliance for Research on Avian Diseases (GARAD) Conference|
|Event Date & Time||On Tue, 30 Jun 2015 at 09:45:00 - 10:00:00|
|Venue||Edmond J. Safra Lecture Theatre|
|Abstract Title||RNA Sequencing Elucidates Large-Scale Temporal Dysregulation of Gene Expression in B19 versus B2 Haplotype Activated Macrophages|
|Affiliations||College of Veterinary Medicine, Western University of Health Sciences, Pomona, CA 91766, USA|
Discovering genetic biomarkers associated with disease resistance and enhanced immunity is critical to developing advanced strategies for controlling viral infections. A greater understanding of the host immune response at the molecular level remains of strategic importance for the development of molecular tools. We have previously demonstrated that B2 haplotype birds resolved infectious bronchitis virus (IBV) infection more readily than B19 infected chicks and that B2 macrophages consistently show more vigorous responses to Poly IC and IFNγ stimulation than B19 derived macrophages. We analyzed the results of RNA sequencing of unstimulated and stimulated macrophages from B2 and B19 haplotypes and found significant differences in a large number of genes in several pathways including innate immune pathways, apoptosis and cell organization. Whereas B2 macrophages display tight regulation, B19 macrophages appear to be unable to regulate all of these cellular mechanisms efficiently. The inability of B19 macrophages to successfully activate the innate immune response might directly impact their response to a wide range of pathogens.