Event Title First Global Alliance for Research on Avian Diseases (GARAD) Conference
Session Title Unknown
Event Date & Time On Mon, 29 Jun 2015 at 17:25:00 - 17:45:00
Venue Edmond J. Safra Lecture Theatre
Abstract Title Identification of new receptor of avian leukosis virus J with DF1 cell line expressing the viral envelope protein
Authors Aijian Qin
Affiliations College of Veterinary Medicine, Yangzhou University
Abstract Content

The viral cell receptor is an attractive target for anti-viral strategies, and the identification of viral cell receptor is critical for better understanding and controlling viral disease. Here, we developed a DF1 cell line expressed viral envelope protein of Avian leukosis virus subgroup J (ALV-J), which could block ALV-J to infect the DF1 cells. With the cell line we identified a chicken Annexin A2 (chANXA2) as a new receptor for ALV-J. The antibodies or siRNA to chANXA2 significantly inhibited ALV-J infection and replication, and over-expression of chANXA2 permitted the entry of ALV-J into its non-permissible cells. Our findings have not only identified chANXA2 as a novel biomarker for anti-ALV-J, but also demonstrated that cell lines with the expression of viral receptor-binding protein could be as efficient tools for isolating functional receptors to identify novel anti-viral targets.